12/26/2023 0 Comments Acorn barnacle![]() ![]() Historically, the majority of research groups have designated these proteins as “cement proteins” based on their location of isolation. During the last three decades, a number of unique proteins in the adhesive have been identified ( Kamino et al., 1996, 2000 Naldrett and Kaplan, 1997 Jonker et al., 2014 Lin et al., 2014 Wang et al., 2015 So et al., 2016, 2017 Rocha et al., 2018 Schultzhaus et al., 2019). This cement is a secreted material ( Dickinson et al., 2009) that transitions to a recalcitrant and permanent solid in the mature form ( Naldrett, 1993 Naldrett and Kaplan, 1997 Kamino et al., 2000). amphitrite.Īs a major marine macrofouler, acorn barnacles adhere to a variety of surfaces via a proteinaceous adhesive, commonly referred to as barnacle cement. While some proteins are clearly enriched at the surface interface, our findings challenge the concept that cement proteins are exclusive to the substrate interface and suggest they may have an expanded physiological role beyond substrate adhesion-related processes of A. ![]() The proteomics analysis also revealed the presence of other proteins first identified in the adhesive layer. Mass spectrometry analysis of proteins recovered from transverse histological sections of the upper portion of barnacles indicates the presence of these same proteins, complementing the immunostaining observations. Additionally, we employ a modified, pressure cycling technology approach to recover protein from histological tissue sections to perform proteomics analysis. Confocal imaging reveals specific staining along different tissue linings of the organism as well as other non-cementing regions. Here, we employ immunological analysis of Amphibalanus amphitrite tissue sections and identify the presence of two cement proteins, AaCP19-1 and AaCP43-1, in areas far removed from the adhesive interface. Recent work has expanded the set of proteins identified within the adhesive interface, but one outstanding question concerns their spatial distribution throughout the organism. Their success is largely due to an ability to adhere to diverse substrates via a sub-micron thick proteinaceous adhesive layer that develops as the organism molts and expands its base. Naval Research Laboratory, Washington, DC, United StatesĪcorn barnacles are major marine fouling organisms. 6Department of Biology, The College of New Jersey, Ewing, NJ, United States.Naval Research Laboratory, Washington, DC, United States 5Center for Bio/Molecular Science and Engineering, U.S. ![]() 4American Society for Engineering Education, Washington, DC, United States.3Naval Research Enterprise Internship Program, American Society for Engineering Education, Washington, DC, United States.2George Mason University, Fairfax, VA, United States.1National Research Council Research Associateship Program, National Academy of Science, Engineering, and Medicine, Washington, DC, United States.Schultzhaus 1†‡ Chenyue Wang 2† Shrey Patel 3 Madeline Smerchansky 3 Daniel Phillips 4‡ Chris R. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |